Temporary

Part:BBa_K364101:Design

Designed by: Ophir Keret   Group: iGEM10_Debrecen-Hungary   (2010-07-27)

Zinc Finger Protein AAA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequance of the alpha recognition helix was united to stabilizing domain on both N and C termini (for more information see BBF RFC ??- In silico design of DNA binding domains from zinc finger polymers).
The nucleotide sequence was then extracted by using the website [http://baderlab.bme.jhu.edu/cgi-bin/gd/gdRevTrans.cgi gene design reverse tranlation function]. The next step was making the parts compatible with biobrick standard RFC25 by removing the appopriate restriction enzyme sites using the website [http://baderlab.bme.jhu.edu/cgi-bin/gd/gdSSRem.cgi gene design restrition enzyme site subtraction].
Data (restriction site removal and retranslation fidelity) was rechecked with the aid of Serial Cloner software and uploaded to the parts registry as a basic part.

Source

Zinc fingers amino acid sequence was adapted from [http://zincfingertools.org zinc finger tools].

References

1. [http://nar.oxfordjournals.org/cgi/content/full/34/suppl_2/W516 Zinc Finger Tools: custom DNA-binding domains for transcription factors and nucleases] Mandell JG, Barbas CF 3rd. Nucleic Acids Res. 2006 Jul 1;34 (Web Server issue):W516-23.]

2. [http://www.ncbi.nlm.nih.gov/pubmed/11340073?dopt=Abstract Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors] Dreier B, Beerli RR, Segal DJ, Flippin JD, Barbas CF 3rd. . J Biol Chem. 2001 Aug 3;276(31):29466-78.

3. [http://en.wikipedia.org/wiki/Zinc_fingers Zinc finger entry] adapted from Wikipedia, the free encyclopedia